Chiral column chromatography is a variant of column chromatography in which the stationary phase contains a single enantiomer of a chiral compound rather than being achiral. The two enantiomers of the same analyte compound differ in affinity to the single-enantiomer stationary phase and therefore they exit the column at different times. The chiral stationary phases consist of a suitable chiral compound attached to the surface of an achiral support such as silica gel, which creates a Chiral Stationary Phase (CSP).
The most common chiral stationary phases are based on oligosaccharides such as Cellulose or Amylose, as well as Immobilized Polysaccharide CSPs.
Other common chiral stationary phases are Cyclodextrin, Protein, Enzyme, Crown Ether, Anion-Exchange, Ligand Exchange, Pirkle etc.