CentriPure Column Array
Hydrated Gel Filtration Column Array for Protein Purification and Desalting
CentriPure P96 Gel Filtration Column Arrays are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from antibodies, enzymes and other proteins larger than 5 kD. The gel matrix of CentriPure is Zetadex-25, a beaded composite material composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability.
Molecules purified with Zetadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal. The size exclusion cut-off for Zetadex-25 is 5 kD for proteins. Proteins larger than 5 kD in a typical sample volume of between 150 to 300 µL can be processed with an elution volume of 300 to 500µL.