HALO 90Å Fused-Core®

HALO column packings are not made the typical way. Instead, the particles packed into HALO® columns are manufactured using Fused-Core® particle technology that was specially developed to deliver hyper-fast chromatographic separations while avoiding the reliability issues so often associated with fast HPLC.

  1. HALO 2 µm columns will deliver reliable high speed and high resolution separations at pressures lower than non-core sub-2 µm columns.
  2. HALO 2.7 µm columns can meet or exceed the performance of most non-core sub-2 µm columns at pressures one-third to one-half the back pressure under the same conditions.
  3. HALO 5 µm columns match the performance of totally porous 3 µm columns at roughly half the back pressure under the same conditions.

Early Explanations for Superior Performance

• Faster Mass Transfer due to a thin porous bonded-phase layer exterior to particle’s solid silica core
• More Uniform and Stable Column beds due to very narrow particle

 

Their ability to generate hyper-fast separations comes not only from their small particle size (2.7 µm) but also from the unique Fused-Core® particle technology that creates a 0.5 µm porous shell fused to a solid core particle. As you increase mobile phase flow rate to speed-up a separation, the slow mass transfer of solute molecules inside the particles can limit resolving power. Fused-Core® particle technology addresses this limitation by providing an incredibly small path (0.5 µm) for diffusion of solutes into and out of the particles, thereby reducing the time solute molecules spend inside the particles and minimizing a major barrier to fast chromatographic separations.

 

HALO 90Å for small molecules < 5000 Da

 

 
 
FOR LARGE MOLECULES PLEASE REFER TO HALO®BioClass
 
 
Guidance for Pore Size Selection
 
MW Range Pore Size (Å)

 

Column Family 

 

HALO Product
< 5000 Da 90 HALO 90Å C18, AQ-C18, C8, Phenyl-Hexyl, Biphenyl, PFP, ES-CN, RP-Amide, HILIC, Penta-HILIC 
< 20 kDa* 90 HALO BioClass HALO Glycan*
100 Da < MW < 15kDa 160 HALO BioClass HALO Peptide (ES-C18, ES-CN, Phenyl-Hexyl)
2kDa < MW < 500kDa 400 HALO BioClass HALO Protein (ES-C18, C4)
> 50kDa 1000 HALO BioClass HALO Protein (ES-C18, C4)
*for glycans, glycopeptides and glycoproteins
 
 
 
 
 

Halo UHPLC guard column

 

 

  • Ultra-low dispersity for UHPLC columns
  • Effectively filters out particulate material and prevents column inlet frit plugging
  • Collects non-eluting compounds from the sample and prevents column fouling.
  • Finger-tight guard replacement design
  • Easily replace guard cartridge without removing guard holder from the flow path

Auto-adjusting ZDV fitting ensures optimum connection to the HALO analytical column

Simple adjustment to fit any UHPLC column

 

Halo® UHPLC guard columns are finger-tight, direct-connect units that represent the latest in fittings and column protection technology.  They are easy to use, maintain ultra-low peak dispersion and are safe to operate at pressure up to 600 psi.

 

HALO C18 Care & Use
HALO C8 Care & Use
HALO HILIC Care & Use
HALO PFP Care & Use
HALO Phenyl-Hexyl Care & Use
HALO RP-Amide Care & Use

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