HALO 90Å Fused-Core®
HALO column packings are not made the typical way. Instead, the particles packed into HALO® columns are manufactured using Fused-Core® particle technology that was specially developed to deliver hyper-fast chromatographic separations while avoiding the reliability issues so often associated with fast HPLC.
- HALO 2 µm columns will deliver reliable high speed and high resolution separations at pressures lower than non-core sub-2 µm columns.
- HALO 2.7 µm columns can meet or exceed the performance of most non-core sub-2 µm columns at pressures one-third to one-half the back pressure under the same conditions.
- HALO 5 µm columns match the performance of totally porous 3 µm columns at roughly half the back pressure under the same conditions.
Early Explanations for Superior Performance
• Faster Mass Transfer due to a thin porous bonded-phase layer exterior to particle’s solid silica core
• More Uniform and Stable Column beds due to very narrow particle
Their ability to generate hyper-fast separations comes not only from their small particle size (2.7 µm) but also from the unique Fused-Core® particle technology that creates a 0.5 µm porous shell fused to a solid core particle. As you increase mobile phase flow rate to speed-up a separation, the slow mass transfer of solute molecules inside the particles can limit resolving power. Fused-Core® particle technology addresses this limitation by providing an incredibly small path (0.5 µm) for diffusion of solutes into and out of the particles, thereby reducing the time solute molecules spend inside the particles and minimizing a major barrier to fast chromatographic separations.
HALO 90Å for small molecules < 5000 Da
FOR LARGE MOLECULES PLEASE REFER TO HALO®BioClass
Guidance for Pore Size Selection
| MW Range | Pore Size (Å) |
Column Family
|
HALO Product |
| < 5000 Da | 90 | HALO 90Å | C18, AQ-C18, C8, Phenyl-Hexyl, Biphenyl, PFP, ES-CN, RP-Amide, HILIC, Penta-HILIC |
| < 20 kDa* | 90 | HALO BioClass | HALO Glycan* |
| 100 Da < MW < 15kDa | 160 | HALO BioClass | HALO Peptide (ES-C18, ES-CN, Phenyl-Hexyl) |
| 2kDa < MW < 500kDa | 400 | HALO BioClass | HALO Protein (ES-C18, C4) |
| > 50kDa | 1000 | HALO BioClass | HALO Protein (ES-C18, C4) |
- Ultra-low dispersity for UHPLC columns
- Effectively filters out particulate material and prevents column inlet frit plugging
- Collects non-eluting compounds from the sample and prevents column fouling.
- Finger-tight guard replacement design
- Easily replace guard cartridge without removing guard holder from the flow path
Auto-adjusting ZDV fitting ensures optimum connection to the HALO analytical column
Simple adjustment to fit any UHPLC column
Halo® UHPLC guard columns are finger-tight, direct-connect units that represent the latest in fittings and column protection technology. They are easy to use, maintain ultra-low peak dispersion and are safe to operate at pressure up to 600 psi.
HALO C18 Care & Use
HALO C8 Care & Use
HALO HILIC Care & Use
HALO PFP Care & Use
HALO Phenyl-Hexyl Care & Use
HALO RP-Amide Care & Use
