NUCLEOGEL®

Sulfonated spherical PS/DVB resins in different ionic forms: mean particle size 10 µm, pore size 100 Å. Separation mechanism includes steric exclusion, ligand exchange and partition effects, ligand exchange being the predominant force, since the hydrated metal ions form strong interactions with the hydroxyl groups of the sample molecules. The intensity of these interactions decreases in the sequence Pb, Ca, Na.

NUCLEOGEL® Ion exchange chromatography columns for biological macromolecules.

Separation mechanism includes steric exclusion, ligand exchange and partition effects, ligand exchange being the predominant force, since the hydrated metal ions form strong interactions with the hydroxyl groups of the sample molecules. The intensity of these interactions decreases in the sequence Pb, Ca, Na.

NUCLEOGEL® SAX

Polymer-based HPLC phases for anion exchange chromatography of biological macromolecules 

Features

  • Polymer-based strongly basic anion exchanger -N+(CH3)3, gel matrix quaternized PEI;
  • Particle size 8 µm, pore size 1000 Å · USP L23
  • pH working range 1–13, max. working pressure 200 bar
  • Eluent in column 0.1 M Na2SO4 + 0.2 % NaN3 
  • Recommended application: purification of peptides, large proteins and oligonucleotides, high capacity for proteins even at pH 10 

NUCLEOGEL® SCX 
Polymer-based HPLC phase for cation exchange chromatography of biological macromolecules 

Features

  • Polymer-based strongly acidic cation exchanger -SO3–, hydrophilic gel matrix
  • Particle size 8 µm, pore size 1000 Å · USP L22
  • pH working range 1–13, max. working pressure 200 bar
  • Eluent in column 0.1 M Na2SO4 + 0.2 % NaN3 
  • Recommended application: proteins, peptides and carbohydrates with high isoelectric point


NUCLEOGEL® SUGAR 810 
Polymer-based HPLC phases for separation of sugars 

Features

  • Sulfonated polystyrene / divinylbenzene resins in different ionic forms
  • Due to a different selectivity pattern compared to NUCLEOGEL® SUGAR columns, the range of application is considerably enlarged
  • Separation mechanism includes ion exclusion, ion exchange, size exclusion, ligand exchange as well as NP and RP chromatography 
  • H+ form: Separation of sugars, sugar alcohols and organic acids · USP L17
  • Ca2+ form: Separation of mono-, di- and oligosaccharides · USP L19

 

NUCLEOGEL® SUGAR and NUCLEOGEL® ION 300 OA 
Polymer-based HPLC phases for separation of sugars 

Features 

  • Sulfonated spherical PS/DVB resins in different ionic forms; mean particle size 10 µm, pore size 100 Å
  • Separation mechanism includes steric exclusion, ligand exchange and partition effects, ligand exchange being the predominant force, since the hydrated metal ions form strong interactions with the hydroxyl groups of the sample molecules. The intensity of these interactions decreases in the sequence Pb, Ca, Na


NUCLEOGEL® ION 300 OA: H+ form for separation of sugars, alcohols and organic acids · USP L17
NUCLEOGEL® SUGAR Ca: separation of mono- and oligosaccharides, sugar alcohols · USP L19
NUCLEOGEL® SUGAR Pb: separation of mono- and disaccharides from food & biological samples · USP L34
NUCLEOGEL® SUGAR Na: separation of oligosaccharides from starch hydrolyzates and food · USP L58

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