Technical Information, Chiral

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Chiral Technologies - Chiral Analytical HPLC Columns

Information on Immobilized Chiral Phases under construction!

Chiral Phases:

• spherical porous silica
• reversed-phase applications
• unique separation characteristics
• 150+ cited references
• over 200 chiral molecules resolved

Phase	Chiral selector	Particle size (µm)	Application
Chiral-AGP	acid glycoprotein	5	broad: amines; acids; alcohols; esters; sulphoxides
Chiral-CBH	cellobiohydrolase	5	alcohols; phenols; carbonyls; amides; ethers; esters
Chiral-HSA	human serum albumin	5	weak acids; strong acids; zwitterionic compounds

Method development:
Solution pH, organic solvent modifier & aqueous buffer affect the performance of the columns

Parameter	Typical conditions	Effect of varying parameter
pH	4 - 7	variable
buffer	phosphate 0.01 - 0.1M	increase in buffer concentration can increase retention& enantioselectivity
organic solvent	propan-2-ol, acetonitrile, methanol 5-15%	Selection of solvent strongly affects enantioselectivity. Higher organic solvent ratios reduce retention time for all phases. For both AGP & HSA columns, enantioselectivity is simultaneously reduced, whilst for CBH columns it is often increased.

Ordering Information:
Semi prep columns available without discount

Phase	Column dimensions
Phase	100 x 2	150 x 2	100 x 3	150 x 3	50 x 4	100 x 4	150 x 4
Chiral-AGP	AGP100.2	AGP150.2	AGP100.3	AGP150.3	AGP50.4	AGP100.4	AGP150.4
Chiral-CBH	CBH100.2	CBH150.2	CBH100.3	CBH150.3	CBH50.4	CBH100.4	CBH150.4
Chiral-HSA	HSA100.2	HSA150.2	HSA100.3	HSA150.3	HSA50.4	HSA100.4	HSA150.4

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